Modified Field stain - rapid viability test for Trichomonas vaginalis

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Modified Field's staining--a rapid stain for Trichomonas vaginalis.

Trichomonas vaginalis, a flagellate protozoan parasite commonly found in the human genitourinary tract, is transmitted primarily by sexual intercourse. Diagnosis is usually by in vitro culture method and staining with Giemsa stain. There are laboratories that use Gram stain as well. We compared the use of modified Field's (MF), Giemsa, and Gram stains on 2 axenic and xenic isolates of T. vagina...

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The ability of Amies gel agar transport medium to maintain the viability of Trichomonas vaginalis was determined by comparing transported vaginal specimens to specimens immediately inoculated into culture medium. The prevalence of trichomonosis in the study population was 26% (68 of 260 women). The immediate inoculation method detected infections in 64 of 68 infected women (sensitivity of 94.1%...

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Recent Advances in the Field Trichomonas vaginalis

The microaerophilic protist parasite is occurring globally Trichomonas vaginalis and causes infections in the urogenital tract in humans, a condition termed trichomoniasis. In fact, trichomoniasis is the most prevalent non-viral sexually transmitted disease with more than 250 million people infected every year. Although trichomoniasis is not life threatening in itself, it can be debilitating an...

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Viability of Trichomonas vaginalis in vitro at four temperatures.

The effect of temperature as a possible factor on the survival of Trichomonas vaginalis for shipment or routine laboratory maintenance was studied. Ten strains of T. vaginalis, ATCC 30001, ATCC 30238, and eight clinical isolates, were examined for viability when kept incubated at 37 degrees C or removed from this temperature and held at 42, 22, or 5 degrees C for increasing lengths of time with...

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ژورنال

عنوان ژورنال: Journal of Applied Microbiology

سال: 2011

ISSN: 1364-5072

DOI: 10.1111/j.1365-2672.2011.05185.x